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Amino-Acid Homopolymers Occurring in Nature by Munenori Takehara, Hideo Hirohara (auth.), Yoshimitsu Hamano PDF

By Munenori Takehara, Hideo Hirohara (auth.), Yoshimitsu Hamano (eds.)

ISBN-10: 3642124526

ISBN-13: 9783642124525

Microorganisms are able to generating a wide selection of biopolymers. Homopolymer peptides, that are made of just a unmarried kind of amino acid, are some distance much less ubiquitous. the one amino-acid homopolymers identified to take place in nature are awarded during this quantity. Poly-epsilon-L-lysine is a polycationic peptide and shows antimicrobial job opposed to a large spectrum of microorganisms. it's either secure and biodegradable and is as a result used as a nutrients preservative in different international locations. additionally, there was nice curiosity in scientific and different functions of poly-lysine and its derivatives. by contrast, poly-gamma-glutamic acid is an strange anionic polypeptide. it's also water soluble, biodegradable, safe to eat, non-toxic and non-immunogenic and will be chemically transformed to introduce a number of medications. those gains are very worthwhile for pharmaceutical and biomedical purposes. Poly-glutamic acid can be a hugely appealing as nutrition ingredient.

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38 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 Abstract Streptomyces albulus NBRC14147 (previously named IFO14147) is known to produce the amino-acid homopolymer antibiotic, poly-e-L-lysine (e-polyL-lysine, e-PL), consisting of 25–35 L-lysine residues with a linkage between the a-carboxyl group and the e-amino group. Because e-PL exhibits antimicrobial activity against a wide spectrum of microorganisms, including Gram-positive and Gram-negative bacteria, as well as antiphage activity, and because it is both safe and biodegradable, e-PL has been introduced as a food preservative in Japan, South Korea, Y.

56 5 Conclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Abstract Poly-e-L-lysine (e-poly-L-lysine, e-PL) is used as a food additive on the basis of its strong antimicrobial activity (Fine chem 29:18–25, 2000). e-PL is industrially produced by fermentation process using Streptomyces albulus.

3 PEG-Mediated Transformation of S. albulus CR1 Protoplast with pLAE001 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Conjugal Transfer of the oriT-Vector, pLAE003, from E. coli to S. albulus CR1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Construction of a Genetically Engineered Strain of S. albulus CR1 for e-PL Overproduction . . . . . . . . . .

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Amino-Acid Homopolymers Occurring in Nature by Munenori Takehara, Hideo Hirohara (auth.), Yoshimitsu Hamano (eds.)


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