By D. Perlman (ed.)
Meant for researchers in utilized microbiology and environmental engineers, this ebook covers such themes as environmental review of biotechnological tactics and microbial adjustments of haloaromatic and haloaliphatic compounds.
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All were pink facultative methylotrophs that contained hydroxypyruvate reductase and lacked hexulose phosphate synthase. Isolate 4P is a type I methylotroph that lacks hydroxypyruvate reductase. DNA was isolated from M . The specific activity was approximately 180,000 cpm/pg. Sheared radioactive M . Oll p g ) was mixed with unlabeled DNA from the organisms indicated. 14 M phosphate buffer. The mixtures were heated at 100°C for 10 min and then at 65°C until a Cl,thvalue of 100 (Britten and Kohne, 1968)was reached ( 2 4 4 8 hours).
Trotsenko (1975) was similarly successful in isolating 100 colonies of obligate methylotrophs using a modification of the technique of Whittenbury et al. (1970a). 5 ml of a mineralsalts medium and incubated standing with a 1:1 mixture of methane and air at 30°C. Dilutions of enrichments were used to inoculate media solidified with silica gel (Galchenko, 1975). Microcolonies were picked from silica gel plates with the aid of a microscope and transferred to silica gel slopes. The use of silica gel rather than agar has been reported to be more effective because of its reduced toxicity for methylotrophs (Aiyer, 1920; Naguib and Overbeck, 1970) and because the number of colonies of non-methaneutilizing bacteria on the plates was reduced (Galchenko, 1975).
Parasitend. , Abt. 1 22, 311. , and Ohle, W. (1964). , Int. Ver. Theor. Angew. Limnol. 15, 535. Panganiban, A. T. (1976). Abstr. Annu. Meet. Am. Soc. Microbiol. p. 121. Panganiban, A. , Patt, T. , and Hanson, R. S. (1979). AppZ. Enuiron. Microbiol. 37, 303. Patel, R. , Hou, C. , Felix, A. (1978). J. Bacteriol. 133, 352. Patt, T. , and Hanson, R. S. (1978). J. Bacteriol. 134, 636. Patt, T. , Cole, G. , and Hanson, R. S. J . Bacteriol. 120, 955. Patt, T. , Cole, G. , and Hanson, R. S. (1975). In “Microbial Production and Utilization of Gases” (H.
Advances in Applied Microbiology, Vol. 26 by D. Perlman (ed.)